Melatonin promotes the survival of random skin flaps in macaques. (IMAGE)
Caption
Melatonin promotes the survival of random skin flaps in macaques. A skin flap wound model was developed on the back of macaques. The macaques received exogenous melatonin treatment for seven continuous days, with the control group administered an equivalent dose of saline. (a) Schematic showing the design of a 3 × 9 cm flap based on the anterior superior iliac spine on the dorsum of each macaque. (b) Gross appearance of flaps on days 0, 3, and 7 after treatment with saline or melatonin. (c) Quantification of the survival area on day 7 (n = 3 per group). (d) Representative H&E-stained images of saline- or melatonin-treated flaps. (e) Representative images of TUNEL staining indicating cell death in macaque skin tissues treated with saline or melatonin. Scale bar: 200 μm; 100 μm. (f–h) Representative fluorescence immunostaining for CD31 (red), E-cadherin (green), and MMP9 (green) within macaque flaps. Scale bar: 200 μm. The data are presented as the means ± SEM, and Student’s t-tests were performed for (c) comparisons. Ctrl control, Mel melatonin, TUNEL terminal dUTP nick end labeling, MMP9 matrix metallopeptidase 9.
Credit
Burns & Trauma
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CC BY