Figure 1. Identification of aph(3) and its role in streptomycin resistance in Clavibacter michiganensis. (IMAGE)

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Figure 1. Identification of aph(3) and its role in streptomycin resistance in Clavibacter michiganensis.

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(A) Physical map of the aph(3) gene derived from de novo sequencing data. (B) Minimum inhibitory concentrations (MICs) of streptomycin against plasmid-cured strains of C. michiganensis. (C) MICs of streptomycin against C. michiganensis strains with different levels of aph(3) expression. (D) LC-MS analysis of streptomycin standard. (E) Extracted ion chromatogram (EIC) of APH(3)-treated samples at m/z 582.2738. (F) LC-MS detection of phosphorylated streptomycin in APH(3)-treated samples.

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mLife

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