scRNA-seq analysis revealed that POP altered aortic cell heterogeneity. (IMAGE)
Caption
(a) Workflow for single-cell RNA transcriptome experiments. (b) t-SNE visualization displayed (i) 20 minor and (ii) five major clusters in (iii) two groups. (c) Dot plot of five major cell markers. (d) Heatmap of differentially expressed genes in each subpopulation. The proportions of major clusters in the Con and POP groups were shown in (e) a stacked bar plot and (f) a three dimensional pie chart. (g) Ligand–receptor pair numbers per cell type in Con and POP groups. (h) Interaction intensity between myeloid cell and other cell types in the Con and POP groups. (i) Bubble chart depicting markedly elevated ligand–receptor interactions between source and target cells, color-coded by experimental group. The selected ligand–receptor pairs orchestrating myeloid-to-nonmyeloid cellular crosstalk in the Con and POP groups.
Credit
Qinjun Zhang, Weisu Huang, Cheng Chen, Jianfu Shen, Baiyi Lu, Peiwu Li
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND