Comparison of the substrate-binding pockets of CaOMTs. (IMAGE)

Nanjing Agricultural University The Academy of Science

Comparison of the substrate-binding pockets of CaOMTs.

Caption

Comparison of the substrate-binding pockets of CaOMTs. (A) Comparison of differential sites among CaOMT1, CaOMT2, and CaOMT3. (B) Conversion rate of CaOMT3 mutants with quercetin as the substrate. Three biologically independent samples were tested (n = 3). All data represent the mean ± SD of three replicates. Student’s t-test: *p < 0.05; *p* < 0.01; *p < 0.001. (C) Sequence alignment of CaOMTs showing a 25-amino-acid deletion and key residues in CaOMT4. (D) Overall structural comparison of the three-dimensional protein structures of CaOMT3 and CaOMT4. (E) Surface representation of the substrate-binding pocket of CaOMT4 in complex with quercetin. (F) Surface representation of the substrate-binding pocket of CaOMT3 in complex with quercetin. (G) Comparison of amino acid residues surrounding quercetin within the binding pockets of CaOMT3 and CaOMT4.

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Horticulture Research

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