Ectopic SERHL expression repressed gastric cancer cell invasion and metastasis. (IMAGE)
Caption
(A) Overexpression (A1) or knockdown (A2) of SERHL in transfected AGS and MKN-74 cell lines were analyzed by quantitative PCR. ∗∗∗∗P < 0.001 for Control vs. SERHL; ∗∗∗P < 0.01 and ∗∗∗∗P < 0.001 for dCas9-control vs. dCas9-SERHL. (B1/2) Transwell assays revealed the effects of SERHL on the migration of AGS and MKN-74 cells. ∗∗∗∗P < 0.001 for Control vs. SERHL; ∗∗∗P < 0.01 and ∗∗∗∗P < 0.001 for dCas9-control vs. dCas9-SERHL. Data represent mean ± standard deviation. (C) Gastric cancer cell motility was detected using the wound healing assay, with bar graphs on the right quantifying their mobility. ∗∗P < 0.05, ∗∗∗P < 0.01, and ∗∗∗∗P < 0.001. (D1/2) White light and fluorescence images showed tumor metastases forming in the lungs of nude mice after tail vein injection (D1). Quantification results of the lung metastatic foci area were presented by scatter plots (D2). ∗∗∗∗P < 0.001. (E1/2) Typical pictures of the hematoxylin-eosin and immunohistochemical staining in Vector and SERHL groups of nude mice lungs (E1). The immunoreactive score (IRS) was compared between different groups (E2). ∗∗∗P < 0.01. Scale bars, 100 μm. (F, G) The analysis of AKT/mTOR signaling pathway in SERHL overexpression (F) or knockdown (G) gastric cancer cells. AKT pathway inhibitor LY294002 was used to inhibit AKT/mTOR activation.
Credit
Linjie Hong, Siyang Peng, Yidong Chen, Yanci Xie, Yuting Lei, Jieke Wu, Xiaodong Huang, Xiangyang Wei, Ping Yang, Jieming Zhang, Qiong Yang, Weimei Tang, Xiaosheng Wu, Ying Peng, Zhizhao Lin, AiminLi, Side Liu, Jiaying Li, Weiyu Dai, Jide Wang
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