DNA Double-Strand Break by the Endonuclease I-DmoI (VIDEO)

Centro Nacional de Investigaciones Oncológicas (CNIO)

Caption

The video illustrates how the I-DmoI endonuclease nicks the phosphodiester bonds that maintain the structural backbone of the DNA. Based on the initial state (State 1), we can observe: two consecutive metals enter the Active Site (State 2), and a third metal, arrives to the middle position (State 3). The third metal plays an important role in the breakage of the phosphodiester bonds (States 4 and 5). Finally, following the DNA double chain break, the innermost metal leaves the central position (State 6).

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