Figure 3 (IMAGE) Kobe University Caption A: Open Field Test Results. When a mouse is placed in a box, it will begin to investigate its environment. Mice usually prefer corners and avoid central, open areas when in a new environment. By calculating the time spent in each area and the distance moved, it is possible to evaluate the mouse's level of anxiety. The 15q dup mice used in this research up until now displayed a high level of anxiety in this experiment. B: Social Interaction Test Results. Two mice that have never encountered each other before are placed in the same box. Sociability is evaluated by observing how long it takes them to interact and their physical contact. In the research group's studies up until now, 15q dup mice's sociability during this test was lower than that of normal mice. C: Results of the Reverse Learning Test using a Barnes maze: A Barnes maze is a circular plate with 12 holes and only one of the holes is the 'target'. The target hole has a box below it that the mouse can enter. A bright light is positioned above the circular plate. Mice generally dislike bright light and prefer dark places, so if a mouse is put on the plate, it will look for somewhere to hide. Using this characteristic, the mouse can be made to learn the target hole's location over a period of days (C, upper illustration). Once the mouse has learned the target's location correctly, the target's location is reversed and relearned (C, lower illustration). It is understood that normal mice learn the new target location comparatively quickly compared to 15q dup mice who are slow to learn the new target, and persist in going to the original target's location (perseveration). For the learning test, the target is removed and learning is evaluated by observing which hole(s) the mouse goes to and how long it stays there (Fig.3- right: TA= the new target hole and the 2 holes on either side. AJ1, AJ2: The 3 holes in each adjacent sector, OP: The previously learned target, and the 2 holes on either side.). Credit Takumi et al., Nature Communications, 2021 Usage Restrictions Please credit License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.