Photonic Resonance Energy Transfer (IMAGE)
Caption
Photonic resonance energy transfer described by Förster, or FRET, occurs when two small proteins come within a very small distance of each other -- eight nanometers or less. The fluorescence lifetime of the donor molecule will become shorter -- from 3 nanosecond to, perhaps, 2.5 nanoseconds. We then interpret this as evidence that the two proteins of interest are physically interacting with each other -- a molecular signaling event.
Credit
Akira Chiba/University of Miami
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