Broca (IMAGE)
Caption
To confirm if the increased nestin+ neurons in the medial septum-diagonal band of Broca came from the differentiation of immature cells, the rats were injected with BrdU after olfactory nerve transection. Using double fuorescent immunocytochemistry for nestin and BrdU, nestin+ neurons in the olfactory nerve transection group showed clear nestin immunoreactivity in cell bodies. Clear BrdU immunoreactivity was visible in nuclei labeled by red fuorescence in the medial septum-diagonal band of Broca (Figure 4B). However, nestin+/BrdU+ neurons were not observed in cells of sagittal or coronal slices.
Credit
<i>Neural Regeneration Research</i>
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