BOLORAMIS (IMAGE)

Wyss Institute for Biologically Inspired Engineering at Harvard

BOLORAMIS

Caption

The researchers compared the BOLORAMIS method with single-molecule FISH (smFISH) analysis, a common standard for RNA localization studies, by tracing the location of a long non-coding RNA molecule known as MALAT1. Whereas, smFISH analysis detects MALAT1 in a rather diffuse pattern, BOLORAMIS resolves individual MALAT1 sites in the cells (orange in the left images) as small punctae, and shows that the RNA shuttle between the cytoplasm (top) and nucleus (bottom) in cells. The nuclei are stained blue in all fluorescent images on the left, while the images on the right show where in cells' overall structures MALAT1 resides.

Credit

Wyss Institute at Harvard University

Usage Restrictions

None

License

Licensed content

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.