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Niigata University

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The optical flow (OF) algorithm was applied to the image segmentation and analysis of first passaged (p1) oral keratinocyte cultures to assess overall cell/colony growth and motion speed. The mean motion speed (MMS) of p1 cells was monitored as an indicator of proliferative capacity, and the MMS threshold required for the transfer of these cells to 3D culture conditions was determined by calculating the population doubling time. The MMS was also used as an index to predict the epithelial regenerative capacity by evaluating histologic features of the oral keratinocytes after receiving experimental metabolic challenge during 2D culture conditions.

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Niigata University

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