Schematic Diagram (IMAGE)
Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS
Caption
This is the Schematic diagram and the performance of skull optical clearing window (SOCW) technique. (a) Four steps: immobilization, skull optical clearing, cortical imaging, and recovery. (b) A custom-built head immobilization device consisting of a skull-holder and a custom-built plate is used to reduce motion artifact during imaging. (c) Anatomical structure of mouse skull. (d) Schematic of the SOCW. A layer of plastic wrap is placed over the cleared skull to separate the water immersion objective from the optical clearing agent (OCA). (e) Representative fluorescence images of the dendrites through the skull, before and after skull optical clearing. We found that the image quality was considerably improved and that it was sufficient for imaging the dendritic spines through the cleared skull. (f) The imaging depth through the SOCW. Orthogonal (x-z) projections of dendrites, before and after skull optical clearing, demonstrating that the depth enhances obviously after clearing (the imaging parameter and data processing were the same). (g-h) The repeatability of SOCW technique. Repeated imaging of the dendrites (g) and spines (h) of Thy1-YFP neurons obtained over 1-d interval. Scale bar, 10 μm.
Credit
Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution. Yan-Jie Zhao, Ting-Ting Yu, Chao Zhang, Zhao Li, Qing-Ming Luo, Tong-Hui Xu & Dan Zhu. <i>Light: Science & Applications</i> volume 7, page 17153 (2018)
doi:10.1038/lsa.2017.153
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