X Ray Diffraction (IMAGE)
Caption
Conventional protein X-ray diffraction images are processed to remove the sharp Bragg reflections, producing noisy images of diffuse intensity (left). Images from multiple crystal orientations are integrated into a 3-D dataset in which the signal is statistically averaged (center, showing a level surface of diffuse intensity in three-dimensions). The 3-D data show greatly enhanced diffuse features, as seen in simulated diffraction images obtained using the integrated data (right, compare to left panel). The 3-D data are used to validate and refine models of protein motions.
Credit
Los Alamos National Laboratory
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