Photoblueing (IMAGE)

University of Würzburg

Photoblueing

Caption

Confocal fluorescence images of glass surfaces coated with the cyanine dyes Alexa Fluor 647 (a) and CF660C (b) and with carborhodamine dye ATTO647N (c) after light excitation at 568 nanometres (nm). By exciting the red-absorbing dyes at 640 nm in certain areas (negative images top right), dyes are photoconverted there and it is possible to write letters on the surface that were excited at 568 nm and fluoresce at about 580 nm. The carborhodamine dye shows more efficient photobluing than the cyanine dyes.

Credit

Team Markus Sauer / University of Wuerzburg

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