Mouse Skin Cells before and after Cell Culture (IMAGE)

BMC (BioMed Central)

Mouse Skin Cells before and after Cell Culture

Caption

Now you see it, now you don't: the epigenetic mark, 5-hydroxymethylcytosine (5hmC), does a disappearing act in culture. Mouse skin cells before and after cell culture show major loss of 5hmC, one of the many rapid molecular changes observed in cell cultures, reported by Nestor and colleagues in Genome Biology. Images depict the loss of red immunostaining for 5hmC in nuclei from cells grown in culture, but not green immunofluorescence (5-methylcytosine) and blue DAPI staining (DNA). Scale bar 5 microns.

Credit

S. Pennings lab.

Usage Restrictions

None

License

Licensed content

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.