IMAGE (IMAGE) Nara Institute of Science and Technology Caption Shootin1a Is Required for Spine Structural Plasticity. Fluorescence time-lapse images of dendritic spines (A) and time course of their volume changes (B) of hippocampal neurons in slice culture. Spines were stimulated by local application of a neurotransmitter glutamate for 30 s (red asterisks). 30-sec stimulation of control spines induced their rapid enlargement which persisted for more than 30 min (blue arrows, top). Downregulation of shootin1a significantly inhibited the spine enlargement (middle). Furthermore, re-addition of shootin1a rescued the reduction of the spine enlargement (blue arrows, bottom), thereby indicating that shootin1a plays an essential role in spine structural plasticity. Credit Naoyuki Inagaki Usage Restrictions Credit required. License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.