Drug Resistance Mutations Also Enhance Growth in Malaria Parasite (IMAGE)
Caption
Gabryszewski used a gene editing technique based on "zinc-finger nucleases" to investigate the gene pfcrt that when mutated makes the malaria parasite Plasmodium falciparum resistant to chloroquine. These authors introduced multiple mutations into parasites cultured in human red blood cells in the laboratory, and characterized a novel set of mutations that make parasites drug resistant and grow even faster than drug-sensitive parasites. The caption shows how the genomic pfcrt sequence was changed into a recombinant form using this gene editing method involving two DNA plasmids that are introduced into the parasite.
Credit
Stanislaw Gabryszewski and David Fidock
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