A principle of PIC technology to detect gene expression from photo-irradiated areas (IMAGE)
Caption
1) A primer with a photocleavable blocker is dropped onto the section. The RNA of the gene is then converted to DNA by the reverse transcription reaction. 2) The blocker is then removed from the primer when the target cells are photo-irradiated. 3) Only the gene from the photo-irradiated areas are amplified and detected.
Credit
Shinya Oki and Yasuyuki Ohkawa
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