Figure 7 (IMAGE) Impact Journals LLC Caption FOXC2 is necessary for the ability of cells that have undergone EMT to augment neoangiogenesis. (A) RFP/luciferase-labeled MCF-7 cells were admixed with either HMLE-Snail-shControl or HMLE-Snail-shFOXC2 cells, and orthotopically implanted into female NOD/SCID mice. The bioluminescent signal emitted by the implanted cells, or the resulting primary tumors, was recorded using bioluminescent imaging 1 day post injection and 8 weeks post implantation, respectively. Blue, least intense, to red, most intense. (B) The tumors from the mice in (A) were excised and imaged prior to processing for histology. Graphical representation of the tumor volumes at endpoint is shown on the right. (C) Tissue sections, from the core regions of the tumors in (B), were stained with H&E. Arrows indicate the location of blood vessels. Scale bar, 100 &mu;m. The average number of blood vessels per microscopic field is plotted on the right. (D) Tissue sections, from the core regions of the tumors in (B), were co-stained with antibodies directed against the SV40 large-T antigen (green) and human CD31 (red). Nuclei were counterstained with DAPI (blue). Right panels are merged images of individual channels. Insets represent high-magnification images of encircled areas. Arrows indicate SV40 large-T antigen/CD31 double-positive cells. Scale bar, 100 &mu;m. n = 5 mice/group. Representative images are shown. Credit Correspondence to - Tapasree Roy Sarkar - firstname.lastname@example.org Usage Restrictions Copyright: © 2021 Sphyris et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.