Figure 5 (IMAGE)
Caption
Effect of 48-hour treatments on proliferation. NFkB protein expression level in THP-1 cell line was determined by Western Blot, starting from 15 μg of total lysates after 48 h of exposure to ELT, DFX and Cytarabine, alone and in combination. The most representative cropped images of blots are displayed. The proteins were detected using Image Studio Digit software and the intensity ratios of immunoblots compared to that of untreated control, taken as 1 (arbitrary unit), were quantified after normalizing with respective loading controls for the housekeeping protein β-Actin. The histogram represents the relative quantification for NFkB expression as mean ± SD of three independent experiments A t-test has been used to evaluate the statistical differences in protein expression levels. * indicates p ≤ 0.05 compared to non-treated sample (NT).
Credit
Correspondence to - Francesca Rossi - francescarossi@unicampania.it
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License
CC BY