Figure 6 (IMAGE)

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Figure 6

Caption

RA inhibits progenitor-like liver cancer cell growth in vitro. (A) Heatmap representation of the relative expression of ADH4, CYP3A4, DKK1, and THY1 progenitor-like gene markers in KYN-2 and HCC-9903 cells measured by qPCR (n = 3). (B) Outline of the effect of RA, RA inverse agonist (BMS-493), and vehicle control (DMSO) on KYN-2 (left) and HCC-9903 (right) in a clonogenic assay. Upper panel: qualitative dot plots comparing the numbers of colony-forming units (CFUs) according to the treatment allocated; Lower panel: respective illustrations of CFUs. (C) Qualitative dot plots of the relative expression of three RA-mediated genes (i.e., ADH1A, ADH1C, and RARB) in KYN-2 (upper panel) and HCC-9903 (lower panel) according to the treatment allocated, measured by qPCR (n = 3). (B, C) Blue: BMS-493; Grey: DMSO; Red: RA. Error bars represent standard deviations. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Credit

Correspondence to - Pascal Pineau - pascal.pineau@pasteur.fr and St&eacute;phane Bertani - stephane.bertani@ird.fr

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Copyright: © 2021 Cerapio et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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