Figure 6 (IMAGE) Impact Journals LLC Caption (A) Following the experimental protocol of Franzetti et al. [11] A673-shA1c cells ?/+ Dox (EWS/Fli1high/EWS/Fli1low) were injected into the tail vein, and metastases were allowed to form in the absence of further Dox treatment (to allow recovery of cell growth in EWS/Fli1low cells). IVIS data at end of study (left) and quantification of photon flux (right); n = 5 for each group; mean and standard error, p-values from two-way ANOVA with repeated measures. (B) Effects of KDM5A (top) and PHF2 (bottom) depletion on metastasis of EWS/Fli1low cells, using the same experimental approach and analysis as in "A"; for KDM5A experiment, n = 10 and 9, respectively, for shControl and shKDM5A; for PHF2 experiment, n = 10 each for shControl and shPHF2. "Metastasis-free" animals were defined as those with total photon flux < 1 × 107. Credit Correspondence to - Paul Jedlicka - paul.jedlicka@cuanschutz.edu Usage Restrictions Copyright: © 2020 McCann et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.