Figure 6 (IMAGE)
Caption
Mislocalization of IQGAP1-BRCA1 in human TNBC tumors phenocopies the dominant mutants and the TNBC cells. (A) Normal mammary tissue: chromogen IHC reveals IQGAP1 membranous localization. (B) Representative of five individual TNBC primary tumor tissue from Caucasian (CA) patients: chromogen IHC reveals IQGAP1 in the nuclear envelop (arrow) and in cytosolic aggregates. (C) Representative of five different TNBC primary tumor tissue from African American (AA) patients: IQGAP1 staining is mainly cytosolic and in aggregates. (D) Representative normal mammary tissue: BRCA1 staining is faint and both nuclear and cytosolic. (E) Representative of five TNBC primary CA tumor tissue: BRCA1 staining in cytosolic aggregates. (F) Representative of five TNBC primary AA tumor tissue: BRCA1 in cytosolic aggregates. (G) Quantitative super-resolution analyses of cytosolic IQGAP1 and BRCA1 in TNBC tumors: triple staining of IQGAP1, BRCA1 and the nuclei visualized by super-resolution microscopy (left). IQGAP1 (green) and BRCA1 (red) reside in invasive front (arrow). Quantification of IQGAP1 and BRCA1 in cytosol (orange bars) and in nuclei (blue bars) of TNBC patient tumors (right). Distinct pools of IQGAP1 and BRCA1 are found individually, co-localized (yellow/orange) or partially overlapping (partial overlap). Error bars are the Means± s. d for n = 3, p-value * 0.05, ** 0.01, *** 0.001.
Credit
Correspondence to - Mahasin A. Osman - Mahasin.Osman@UToledo.Edu, Mahasin_Osman@brown.edu.
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