Role of KYP-2047 treatment on apoptosis Immunofluorescence staining for Caspase-3 was performed to examine apoptosis in the lungs (IMAGE)
Caption
Caspase-3 positive staining resulted lower in lung sections from sham group (A) compared to II/R-injured group (B); POP-inhibition mediated by KYP-2047 treatments, at both doses of 2.5 and 5 mg/Kg, significantly lowered the positive staining for Caspase-3 (C and D); (E) Caspase-3 positive score. These data were confirmed by Western Blot analysis (F, (f)). Moreover, through Western Blot analysis, a substantial Bax low expression, related to a notable increase in Bcl-2, was observed in lung samples from controls compared to II/R-injured mice, while treatment with KYP-2047 significantly reduced Bax protein expression, at the same time increasing the anti-apoptotic Bcl-2 protein levels (G and H, see densitometric units score (g) and (h)). Data represent the means of at least three independent experiments. One-way ANOVA followed by Bonferroni post-hoc. ***p < 0.001 versus Sham; #p < 0.05, ##p < 0.01 and ###p < 0.001 versus II/R.
Credit
Correspondence to - Irene Paterniti - ipaterniti@unime.it
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CC BY