Backtracking in RNA Pol II EC Follows a Stepwise Model (IMAGE)

Hong Kong University of Science and Technology

Backtracking in RNA Pol II EC Follows a Stepwise Model

Caption

Representative structures for each of the four metastable states identified by our Markov State Model (MSM; S1-S4) and connected by the top one pathway from the pre-translocation state (S1) to the backtracked state (S4): S1right arrowS2right arrowS3right arrowS4. The equilibrium population for each state (left bottom corner) and the Mean First Passage Time (MFPT) between each transition (beside arrow, unit in μs) are presented, with their corresponding errors given as followings: 10.5±1.5%, 22.4±2.1%, 4.0±0.7% and 63.1±2.8% (equilibrium population); 0.1±0.0?μs, 0.8±0.1?μs, 5.1±3.0?μs, 1.0±0.7?μs, 95.9±42.3?μs and 191.8±69.0?μs (MFPT). The hybrid RNA/DNA chains (red/blue), the Trigger Loop (purple), Bridge helix (green), Rpb1 residue Y836 and Rpb2 residue Y769 (both in grey) are shown. The DNA TN (dG) and its mismatched RNA nucleotide (rG) are highlighted with cyan and orange stick model, respectively.

Credit

Department of Chemistry, HKUST

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