Global Lengthening of Poly(A) Tails During Oogenesis (IMAGE)
Caption
(A) Schematic illustration of late oogenesis and egg activation. Global poly(A) tail lengths are addressed at three different stages: immature oocyte, mature oocyte, and activated egg.
(B) Global distributions of poly(A) tails at three stages. The median poly(A) tail lengths is 60 nt in immature oocytes, 75 nt in mature oocytes, and 73 nt in activated eggs. The result from biological replicates is shown in Supplemental Figure S2D.
(C) Scatter plots showing the changes of poly(A) tail lengths upon late oogenesis and egg activation, respectively. The mean poly(A) tail lengths from two biological replicates were averaged. n = 2. The median of mean poly(A) tail lengths is 58 nt in immature oocytes, 76 nt in mature oocytes, and 70 nt in activated eggs.
(D) Changes of mRNA abundance upon late oogenesis and egg activation measured by RNA sequencing (RNA-seq).
(E) Examples of individual genes. mTAIL-seq tags were plotted in 3-nt-wide bins and then smoothened with a Hanning window (width = 5). The frequency along the Y-axis was normalized by the maximum value at each stage.
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