Triggering the Subtilisin Food Processor (IMAGE)

National Institute of Standards and Technology (NIST)

Triggering the Subtilisin Food Processor

Caption

The sheath (in green at top), with the desired protein linked to it (in yellow at right), first sticks to the engineered enzyme (in blue at bottom left) -- allowing impurities and other proteins to be washed away. Then an infusion of triggering molecules (red dots) causes the enzyme to cut away the desired protein (at the point where the sheath and protein connect), which can be collected in a pure state. The sheath can then be washed away and the process repeated.

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NIST

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