PARPi-FL imaging of a clitoral excision – invasion from a vulvar squamous cell carcinoma. (IMAGE)
Caption
PARPi-FL imaging of a clitoral excision – invasion from a vulvar squamous cell carcinoma. Imaging with PARPi-FL was also carried out in one patient harboring a recurrent squamous cell carcinoma of the vulva invading the clitoris. (A) Patient biospecimen ex vivo. The dotted area (1) corresponds to the tumor and the surrounding area (2) corresponds to benign margins. Bottom row: Images acquired with the handheld confocal microscope demonstrated tumor cells with enlarged nuclei that were different in size and arranged in a very disorganized manner. Tissue depth 4 μm (tumor) and 6 μm (normal tissue). (B) H&E staining showed that the tumor was restricted to a small area in the tissue – represented by number 1. PARP1 IHC corroborated the confocal findings since no PARP1 expression was present in the stroma (2) whereas it was present in tumor (1). (C) Quantification of PARPi-FL uptake in tumor and normal tissue. Fluorescence signal was significantly (p < 0.0001) higher in the tumor area when compared to the normal surrounding tissue.
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Image created by Demetrio, PDSF, Jewell, E., Reiner, T., et al., Memorial Sloan Kettering, New York, NY.
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