Effect on THP-1 Cells of Adipocyte-Derived Extracellular Vesicles on Cholesterol Efflux (IMAGE)

Children's National Hospital

Effect on THP-1 Cells of Adipocyte-Derived Extracellular Vesicles on Cholesterol Efflux

Caption

Effect on THP-1 cells of adipocyte-derived extracellular vesicles (EVs) from lean and obese subjects on cholesterol efflux gene expression and cholesterol efflux to media. THP-1 cells were incubated with obese and lean visceral adipocyte-derived EVs for 24 hours. Images of fluorescently labeled THP-1 (blue) macrophages, fluorescently labeled Dil-oxLDL (red), and exosomes (1 μg/mL; unlabeled) VAT tissue exosomes from subjects with obese and lean subjects.

Credit

"Cholesterol efflux alterations in adolescent obesity: role of adipose-derived extracellular vesical microRNAs," published online July 23, 2019 in <em>Journal of Translational Medicine</em>. Matthew D. Barberio, Lora J. Kasselman, Martin P. Playford, Samuel B. Epstein, Heather A. Renna, Madeleine Goldberg, Joshua DeLeon, Iryna Voloshyna, Ashley Barlev, Michael Salama, Sarah C. Ferrante, Evan P. Nadler, Nehal Mehta, Allison B. Reiss and Robert J. Freishtat.

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