A schematic of motor neuron hyperexcitability screening (IMAGE)
Caption
This high-throughput screening system is designed to identify compounds that decrease motor neuron hyperexcitability, a cardinal feature of ALS. Motor neurons derived from patients with ALS are cultured in 384-well plates, and compounds from chemical libraries of small molecules against known molecular targets are added after 3-4 weeks. Live GCaMP imaging, to detect calcium waves indicating neuronal firing, is performed 6 and 24 hours later as a measure of excitability. Compounds identified through the primary screen are then put through a confirmation screen and tested directly for efficacy and toxicity.
Credit
Huang X; et al; Cell Reports 2021 Jun 8.
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