Three-Dimensional Reconstruction of Neuronal Cell Bodies and Branches (IMAGE)

Scripps Research Institute

Three-Dimensional Reconstruction of Neuronal Cell Bodies and Branches

Caption

Confocal analysis at 63x magnification, followed by the three-dimensional reconstruction of neuronal cell bodies and branches. The image shows an example of eYFP and CRF in the same neuron. This rendered isosurface analysis demonstrated the colocalization of CRF immunoreactivity within CeA neurons that also expressed Cre-dependent eYFP and validates the crh-Cre rat as a tool to gain more direct access to CRF neurons to study their functional neuroanatomy.

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George lab

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