Novel Technology Enables Detection of Early-Stage Lung Cancer when Surgical Cure Still Is Possible (IMAGE)
Caption
The steps involved in the electric field-induced capture and release (EFIRM) assay are shown. Initially, allele-specific capture probes are immobilized in a conducting polymer gel at the base of each well in a 96 well plate. Subsequently, a small amount (20 to 30 ncl) of untreated saliva or plasma is added to hybridization fluid and added directly to the well (step 1). Subsequently an alternating electric current is applied causing allele-specific hybridization. In step 2, a biotinylated signal probe is added to each well and another electrically stimulated hybridization is performed. Finally, in step 3 a standard streptavidin/biotin/horse radish peroxidase (HRP) signal amplification is performed causing electrons to be drawn up through the gold electrode and the current in nanoamperes is measured by the EFRIM reader.
Credit
Fang Wei, EZLife Bio
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