Unpredictable Threat Test (IMAGE) Society for Neuroscience Caption Figure 3. Unpredictable threat test and cannulae schematics A. In the unpredictable threat test (n = 6), animals were placed in a familiar environment for 25 mins and played two types of auditory stimuli, a novel auditory cue and an unconditioned stimulus (US) for the 4 unpredictable threat days. Each cue was 20 sec long, 75dB and was presented 12 times with an intertrial interval (ITI) of 40-80 sec. Each mildly aversive US was 0.4 sec long, 117dB and presented 12 times with an ITI of 40-80 sec. The threat of the aversive US was unpredictable as there was no relationship between the order of the cues and USs. On the ambiguous cue probe (5th day), monkeys were presented with a novel, ambiguous 20 sec, 75dB cue presented 12 times with an ITI of 100-180 sec in the same context. No USs were presented. All drug manipulations occur on ambiguous cue probes. The cycle then repeated with the novel cue incorporated into the unpredictable threat training. Credit Zeredo <em>et al</em>., <em>JNeurosci</em> (2019) Usage Restrictions None License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.