The Proteomic Workflow to Label and Enrich Proteins using Saha-Ad and Cb[7] Beads (IMAGE)

Institute for Basic Science

The Proteomic Workflow to Label and Enrich Proteins using Saha-Ad and Cb[7] Beads

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Fig.1: The cell lysate (1.0 mgmL 1) was incubated with SAHA-Ad, and then covalently crosslinked by irradiation with UV light (365 nm). The proteins were precipitated and the free probe removed by methanol washes. The proteins were redissolved and incubated with CB[7] beads for 4 hours at 48C. The beads were washed and the captured proteins were released with BAFc.

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