Fig. 1. Biotinylation of Proteins Interacting with a Protein Fused With AirID (IMAGE)

Ehime University

Fig. 1. Biotinylation of Proteins Interacting with a Protein Fused With AirID

Caption

An ancestoral type enzyme AirID was designed from the sequence of biotinylation enzyme BirA of Escherichia coli by an ancestral enzyme reconstruction algorithm with metagenome data. Since AirID has high biotinylation activity and low cytotoxicity, it is possible to biotinylate a protein that interacts with the target protein by expressing it in cells as a fusion protein with the target protein. By identifying these biotinylated proteins using mass spectrometry analysis, it becomes possible to comprehensively identify proteins that interact with the target protein in cells.

Credit

Tatsuya Sawasaki (Ehime University)

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