CRISPR-Cas9 Binding Checkpoints (VIDEO) University of California - Berkeley This video is under embargo. Please login to access this video. To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Caption The Cas9 enzyme must flex and bend in order to bind to the guide RNA (orange). Once the Cas9-RNA complex finds its target DNA (red), the cutting region of Cas9 (yellow) will swing into place relative to its mate (blue) only when the RNA and DNA correctly match. Only then does the enzyme cut the double-stranded DNA. These detailed movements were revealed by fluorescence experiments reported in a Nature paper from the Doudna lab. Credit Sam Sternberg/UC Berkeley Usage Restrictions None License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.