CRISPR-Cas9 Binding Checkpoints (VIDEO)

University of California - Berkeley

Caption

The Cas9 enzyme must flex and bend in order to bind to the guide RNA (orange). Once the Cas9-RNA complex finds its target DNA (red), the cutting region of Cas9 (yellow) will swing into place relative to its mate (blue) only when the RNA and DNA correctly match. Only then does the enzyme cut the double-stranded DNA. These detailed movements were revealed by fluorescence experiments reported in a Nature paper from the Doudna lab.

Credit

Sam Sternberg/UC Berkeley

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