A Personalized Human Tissue Construct for Vaccine Testing (IMAGE)
Caption
Step 1: A study participant's immune cells (monocytes) are applied to the tissue construct, which consists of a layer of human endothelial cells grown over a 3D network of human proteins. The monocytes migrate down into the protein matrix, mimicking the natural movement of monocytes out of capillaries and into local body tissues. Step 2: A vaccine is added to the construct, which is incubated for 48 hours. Some of the monocytes differentiate to become dendritic cells during this time. Step 3: Mature dendritic cells emerge from the construct, resembling the movement of the body's dendritic cells to the lymphatic (lymph node) system. Step 4: Dendritic cells are harvested from the construct and cultured with T cells from the same study participant to measure immune response.
Credit
Boston Children's Hospital
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