FluoroDOT assay (IMAGE) Washington University in St. Louis Caption FluoroDOT assay images of dendritic cells secreting protein 1 (TNFa, shown in red) and protein 2 (IL-6, shown in yellow) at different time points (from left to right: Unstimulated, stimulated for 30 mins, 1 hour, 2 hours and 3 hours). The nuclei of the cell are shown in blue. The white arrows highlight the cells which are either secreting only one protein or different amounts of the two proteins and different time points. Credit Srikanth Singamaneni/WashU Usage Restrictions Use allowed with attribution License Original content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.