Figure 1. Schematic (a) and mechanism (c) of p53 Intra Q-body. (b) p53-dependent fluorescence signal and (d) microscopy images. (IMAGE)

Tokyo Institute of Technology

Figure 1. Schematic (a) and mechanism (c) of p53 Intra Q-body. (b) p53-dependent fluorescence signal and (d) microscopy images.

Caption

(a) The double labelled fluorescent dye in the antigen fragment-based Q-body is de-quenched on binding with the target antigen, thus displaying fluorescent signaling for visualizing the intracellular target. (b) p53 peptide concentration-dependent variation in fluorescence signal intensity. (c) Q-body displays a high fluorescent signal on binding with the target in cells expressing p53, as compared to the ‘p53’ negative human cells. (d) Confocal microscopy images of HCT116 p53 and SK-BR-3. Cells which do not express p53, i.e., HCT116 p53(-/-) exhibit no TAMRA-based fluorescence while others (including images stained with Hoechst dye for illuminating nucleus and under bright-field to show the cells) display significant fluorescence.

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