A new method to isolate hepatic stellate cells from fatty tumor-bearing livers in both mice and humans (IMAGE)

Osaka Metropolitan University

A new method to isolate hepatic stellate cells from fatty tumor-bearing livers in both mice and humans

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Cold enzymatic treatment and usage of an antibody against CD49a, a reliable HSC surface marker, minimized the generation of heat stress-associated artefacts and allowed the isolation of HSCs with high yield and high purity.

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Naoko Ohtani, Osaka Metropolitan University

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