Phage infection of Mycobacterium smegmatis (VIDEO)
Caption
Infection of Mycobacterium smegmatis by a genetically engineered mutant of phage Fionnbharth. Three steps in the infection process can be seen: 1) A single phage particle binds to the bacterial cell, and is seen as a red dot 0.42 seconds into the video. 2) Two seconds into the movie, green fluorescence is observed where the phage has injected its DNA into the cell. The green fluorescence comes directly from the phage DNA (ignore the bright green dots at the very ends of the cell). Over the next few seconds, the green-labeled DNA forms a zone of phage replication (ZOPR) and spreads throughout the cell. 3) At 6:25 seconds, lysis occurs and the cell explodes. Total time elapsed is about three hours.
Credit
Charles Dulberger
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