Innate lymphoid cells prevent development of meningitis following pulmonary infection (IMAGE)
Caption
Rag2−/−/Il2rg−/− mice were infected intranasally with 105 colony-forming units of Brucella melitensis. At the development of neurologic complications, or at the conclusion of the study (70 days after infection), brains were collected and transversely sectioned for histopathology. A: Slides were stained with hematoxylin and eosin, and representative images [4× objective (left panels) and 10× objective (right panels)] of naïve and infected sections from the caudal cerebral cortex are shown. The arrows indicate lesions of inflammatory cell infiltrate, and the circle indicates perivascular cuffing. B and C: Immunohistochemistry was performed on serial sections to detect myeloperoxidase (MPO; B) or Brucella antigen (C). B and C: Representative images (10× objective) of rostral cerebrum (left panels), cerebellum (middle panels), and caudal cerebrum (right panels) indicate infiltration of MPO-positive cells (B) and Brucella (C) into the meninges. n = 10 Rag2−/−/Il2rg−/− mice (A–C).
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Dr. Charles Moley
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