Round spermatid injection (ROSI) using a spermatid from a male lacking any Y chromosome genes. The movie features selection of the cell, cytoplasm removal and cell injection (51 sec). This material relates to a paper that appeared in the Jan. 29, 2016 issue of Science, published by AAAS. The paper, by Y. Yamauchi at University of Hawaii in Honolulu, HI, and colleagues was titled, "Two genes substitute for the mouse Y chromosome for spermatogenesis and reproduction."
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