A New Vector for Gene Transfer across the Blood Brain Barrier (video) California Institute of Technology Share Print E-Mail Loading video... Caption Using a new selection method reported in Nature Biotechnology, Caltech researchers have evolved the protein shell of a harmless virus, AAV9, so that it can more efficiently cross the blood brain barrier and deliver genes, such as the green fluorescent protein (GFP) to cells throughout the central nervous system. This movie shows green fluorescent protein (GFP) expression in the spinal cord of an adult mouse that received an intravenous injection of the modified virus, AAV-PHP.B, carrying the gene for GFP. The spinal cord sample was rendered transparent for imaging using a whole-body tissue clearing method called PARS CLARITY previously developed by the Gradinaru lab. Credit Ben Deverman and the Gradinaru laboratory/Caltech and Nature Biotechnology Usage Restrictions None Share Print E-Mail Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.