Experimental Procedure for Gene Knockout Using the pKIR Vector (image) Institute of Transformative Bio-Molecules (ITbM), Nagoya University Share Print E-Mail Caption Plant seeds containing Cas9 are marked with a red fluorescent marker to tell which seeds contain Cas9. Using this method, it becomes possible to obtain seeds that have a knockout mutation in their target gene and do not contain Cas9, in the second generation by selection. This whole process takes about three months. Credit ITbM, Nagoya University Usage Restrictions None Share Print E-Mail Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.