This video shows stress fibers bending by the nucleus of a transmigrating effector T cell crossing transcellularly through inflamed HDMVECs. The movie depicts a Hoechst labeled effector T cell crossing through a transcellular route of IL-1β-stimulated HDMVECs expressing RFP-Lifeact . Images were taken 20 sec apart. Elapsed time is designated as h:mm:ss. Bar, 5 μm.