(TOP) Schematic figure of virus injection. A mixture of DRD2-iTango2 viruses including EGFP reporter were injected into left and right NAc areas in DAT-Cre mouse. At the same mouse, AAV-dFlox-ChR2(H134R)-
mCherry was injected to the right side of VTA area to control DA release selectively. Coronal section view of virus injection and fiber optic implantation. Both NAs were injected by iTango2 viruses and an optic fiber was implanted in both hemispheres.
(BOTTOM) Representative confocal images of DRD2-iTango2 expression by blue light (10 s ON and 50 s OFF, one hour). Red, green, and merged channel images were presented at upper, middle, and bottom row, respectively. Note that TdTomato expression is robust in both NAs but EGFP signals are very high only in the right NAc. High magnification images of left and right NAc were presented for