Body weight evolution (A) and food intake (B) were recorded weekly. C: Body composition (fat mass) was assessed by magnetic resonance imaging (EchoMRI) after 12 weeks of HFD. D: i.p. glucose tolerance tests were performed after 6 weeks of HFD. E: Insulin resistance was estimated by homeostatic model assessment of insulin resistance (HOMA-IR) score, calculated after 12 weeks of HFD. F: Intrahepatic neutral lipid content and liver histology were assessed at sacrifice. Representative images of hematoxylin and eosin (H&E) and Oil Red O (ORO) staining on liver sections. Insets show higher magnification. Data are expressed as means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001. Scale bars: 100 mm (Liver Histology); 50 μm (G, insets). Original magnification, ×40 (insets). VEH-Vehicle alone; TAM-Tamoxifen treated.