A, B, Schematics showing experimental strategies for the heterotopic transplantation of nc-ChCs (A) and h-ChCs (B). E17 MGE cells from Nkx2.1-CreER;Ai14 embryos induced at E16 were transplanted into the hippocampus of P1 host animals (A). E16 hippocampal cells from Nkx2.1- CreER;Ai14 embryos induced at E12 were transplanted into the neocortex of P1 host animals (B). C, D, Upper panels show confocal projection images representing the M-L extent of ChC axonal arbors in coronal sections. An h-ChC transplanted into the host neocortex and an nc-ChC transplanted into the host hippocampus are shown in C and D, respectively. Asterisks indicate both ends of axonal arbors. Confocal single optical sections in lower right panels represent enlarged images of boxed areas in upper panels showing axonal varicosities (magenta) apposed to axon initial segments (green) stained with anti-Ankyrin G antibodies. Lower left panels show individual cartridge structures. E, F, 3-D reconstructions of an h-ChC transplanted into the host neocortex (E) and an nc-ChC transplanted into the host hippocampus (F) in coronal sections. Axonal processes are color-coded according to their depth.