Marine Biological Laboratory (MBL) Fellows Patrick La Riviere of University of Chicago, left, and Hari Shroff of the National Institute of Biomedical Imaging and Bioengineering with the diSPIM system (Dual-view Selective Plane Illumination Microscopy) co-developed by Shroff's laboratory and Applied Scientific Instrumentation. Note the two objectives mounted at right angles above the sample. A light sheet is created from one objective and imaged through the other objective. A stack of images is created by moving the light sheet through the sample. Especially useful for live-cell imaging and developmental biology, the system provides rapid 3-D imaging with low phototoxicity to samples.